Journal: Biomaterials Research

Article Title: Biomimetic Gradient Microporous Scaffold with a Triply Periodic Minimal Surface Enhances Osseointegration by Modulating Macrophage Polarization

doi: 10.34133/bmr.0266

Figure Lengend Snippet: In vivo immune modulation evaluation. (A) Immunofluorescence staining of C-C motif chemokine receptor 7 (CCR7, M1, green) and mannose receptor (CD206, M2, red). (B) Quantitative analysis of CCR7 and CD206 expression. Data are presented as mean ± SD ( n = 3). * indicates significant differences (* P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001).

Article Snippet: Rabbit anti-C-C motif chemokine receptor 7 (anti-CCR7) antibody (M1 marker, Boster, China) and rabbit anti-mannose receptor (anti-CD206) antibody (M2 marker, Boster, China) were used to stain the samples, as these are well-known markers for M1 and M2 macrophage phenotypes, respectively.

Techniques: In Vivo, Immunofluorescence, Staining, Expressing

Journal: Bioactive Materials

Article Title: Multifunctional DNA hydrogels with light-triggered gas-therapy and controlled G-Exos release for infected wound healing

doi: 10.1016/j.bioactmat.2025.06.004

Figure Lengend Snippet: Characterization and promoting wound-healing function of G-Exos. (A) Schematic diagram showing the function of exosomes; (B) TEM image and (C) The NTA result of G-Exos; (D) Cell viability of HUVECs and HaCat cells treated with different concentration of G-Exos; The cell proliferation and migration effect of different concentration of G-Exos on (E) HUEVCs and (F) HaCat cells detected by scratch assay; (G) Confocal images of RAW264.7 cells uptaking of G-Exos; (H) The fluorescent images of iNOS and CD206 expression of RAW264.7 cells under different treatments and (I) Semi-quantitative fluorescence of iNOs and CD206 expression of RAW264.7 cells under different treatments; the concentration of (J) IL-6, (K) IL-10, (L) TNF-α secreted by cells after different treatments, data are presented as means ± SD (n = 3), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: CD86-FITC, anti-mouse CD86 and anti-rabbit CD206 antibodies were purchased from Proteintech Group, Inc (Wuhan, China).

Techniques: Concentration Assay, Migration, Wound Healing Assay, Expressing, Fluorescence

Journal: Bioactive Materials

Article Title: Multifunctional DNA hydrogels with light-triggered gas-therapy and controlled G-Exos release for infected wound healing

doi: 10.1016/j.bioactmat.2025.06.004

Figure Lengend Snippet: In vitro proliferative and anti-inflammatory activity of LGAH. (A) Edu fluorescence images of HaCat cells and HUVECs after different treatments and (B) The corresponding Edu positive cell proportion of each group; CD86 and CD206 expression of RAW264.7 cells under different conditions (Dashed box: Cells treated with LPS) detected by (C) Confocal microscope and (D) Flow cytometry, data are presented as means ± SD (n = 3), ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: CD86-FITC, anti-mouse CD86 and anti-rabbit CD206 antibodies were purchased from Proteintech Group, Inc (Wuhan, China).

Techniques: In Vitro, Activity Assay, Fluorescence, Expressing, Microscopy, Flow Cytometry

Journal: Bioactive Materials

Article Title: Multifunctional DNA hydrogels with light-triggered gas-therapy and controlled G-Exos release for infected wound healing

doi: 10.1016/j.bioactmat.2025.06.004

Figure Lengend Snippet: Immunofluorescence staining of different groups showing the expression of (A) CD31 and collagen, (B) iNOs and CD206 in wound tissues; (C) IL-6, (D) TNF–α and (E) IL-10 expression in the wound tissues of different groups detected by immunohistochemistry (G1: PBS; G2: L-Arg; G3: G-Exos; G4: A–hydrogel; G5: A–hydrogel + laser; G6: LGAH; G7: LGAH + laser).

Article Snippet: CD86-FITC, anti-mouse CD86 and anti-rabbit CD206 antibodies were purchased from Proteintech Group, Inc (Wuhan, China).

Techniques: Immunofluorescence, Staining, Expressing, Immunohistochemistry